The structure of extracellular phosphorylated galactans from Sporobolomyces yeasts.

Two strains of SporoboZomyces pecies, NRRL Y-6493 and NRRL Y-6502, produce extracellular, lightly acetylated, phosphorylated galactans when grown on media containing glucose as the carbon source. D-&lactose and D-galactose 6-phosphate were the only carbohydrate components present in strong acid hydrolysates. oc-D-Galactose 1 -phosphate was isolated after vigorous alkaline hydrolysis. Periodate oxidation analyses of intact and deacetylated polymers indicate that approximately equal proportions of a-1,6and a-1,3galactosyl linkages are present. Polymer phosphate occurs exclusively in the form of cy-n-galactopyranosyl-l-phospho6’-galactosyl phosphodiester end groups.